Second thioredoxin from the mud crab Scylla paramamosain: cDNA cloning and mRNA expression analysis
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چکیده
منابع مشابه
Cloning and characterization of the SpLRR cDNA from green mud crab, Scylla paramamosain.
Infectious diseases have seriously inhibited the aquaculture of mud crab Scylla paramamosain in southeastern China. Identification of the immune molecules and characterization of the defense mechanisms will be pivotal to the reduction of these diseases. Available data show that leucine-rich repeat (LRR) proteins play a crucial role in protein-protein interactions, recognition processes or immun...
متن کاملcDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli
Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon. Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predicted molecular mass and pI of approximately 19.2 kDa and 4.5, respectively. Interestingly, mud crab...
متن کاملMolecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain.
The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx1) showing high identity to the first Tr...
متن کاملCharacterization and expression analysis of the prophenoloxidase activating factor from the mud crab Scylla paramamosain.
Prophenoloxidase activating factors (PPAFs) are a group of clip domain serine proteinases that can convert prophenoloxidase (pro-PO) to the active form of phenoloxidase (PO), causing melanization of pathogens. Here, two full-length PPAF cDNAs from Scylla paramamosain (SpPPAF1 and SpPPAF2) were cloned and characterized. The full-length SpPPAF1 cDNA was 1677 bp in length, including a 5'-untransla...
متن کاملMolecular cloning and expression profiles of nitric oxide synthase (NOS) in mud crab Scylla paramamosain.
The importance of the nitric oxide synthase (NOS) gene family is demonstrated by many studies in vertebrates and invertebrates in recent years. However, it keeps unknown of nitric oxide (NO) system and NOS gene family in mud crab Scylla paramamosain, an important cultured commercial crustacean in China and Pacific area. In this report, the cDNA of NOS containing full-length ORF was cloned from ...
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ژورنال
عنوان ژورنال: Journal of Crustacean Biology
سال: 2013
ISSN: 0278-0372,1937-240X
DOI: 10.1163/1937240x-00002167